Molecular Weight of Bacteriophage PBS2 DNA

BaciZZus subtilis Bacteriophage PBS2-induced DNA Polymerase

The DNA polymerase induced by Bacillus subtilis bacteriophage PBS2 (whose DNA contains uracil instead of thymine) has been purified and characterized for its specificity. The enzyme requires a high ionic strength for optimal stability and activity and is sensitive to various anions and to sulfhydryl reagents. Both dUTP and dlTP are incorporated efficiently as substrates and are competitive inhi...

Bacteriophage PBS2-induced inhibition of uracil-containing DNA degradation.

Degradation of uracil-containing DNA by Bacillus subtilis extracts and its inhibition after infection by the uracil-containing DNA phage PBS2 have been investigated to resolve differences between the published reports of Tomita and Takahashi (1975) and Friedberg et al. (1975, 1976). The product of hydrolysis of PBS2 DNA, tritium labeled in its uracil and cytosine residues, is solely uracil and ...

Deoxythymidylate Phosphohydrolase Induced by Bacteriophage PBS2 during Infection of Bacillus

The deoxythymidylate phosphohydrolase (dTMPase) induced by Bacillus subtilis bacteriophage PBS2 (whose DNA contains uracil instead of thymine) has been partially purified and shown to possess deoxyuridylate phosphohydrolase (dUMPase) activity. The similarities of induction period, pH dependence, heat and trypsin inactivation, sulfhydryl reagent and fluoride inhibition, metal ion effects, kineti...

Uracil-DNA glycosylase inhibitor gene of bacteriophage PBS2 encodes a binding protein specific for uracil-DNA glycosylase.

The uracil-DNA glycosylase inhibitor gene (ugi) of the Bacillus subtilis bacteriophage PBS2 has been subcloned to a 720-base pair DNA fragment contained in pZW2-0.7 and its nucleotide sequence determined. Using nucleotide deletion analysis, we have located the cloned ugi gene along with potential regulatory elements. A promoter-like region (-10 and -35 consensus sequences) similar to other B. s...

Molecular weight of bacteriophage PBS 1 deoxyribonucleic acid.